Multiregional evaluation of the SimPlate heterotrophic plate count method compared to the standard plate count agar pour plate method in water.

نویسندگان

  • R W Jackson
  • K Osborne
  • G Barnes
  • C Jolliff
  • D Zamani
  • B Roll
  • A Stillings
  • D Herzog
  • S Cannon
  • S Loveland
چکیده

A new SimPlate heterotrophic plate count (HPC) method (IDEXX Laboratories, Westbrook, Maine) was compared with the pour plate method at 35 degrees C for 48 h. Six laboratories tested a total of 632 water samples. The SimPlate HPC method was found to be equivalent to the pour plate method by regression analysis (r = 0. 95; y = 0.99X + 0.06).

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Heterotrophic Bacteria Count Index in Drinking Water and Possibility of Biofilm Formation in Household Drinking Water Treatment Devices in Sari, Iran

 Background and purpose: Household drinking water treatment devices are becoming commonplace to improve the quality of drinking water, remove odor, taste, turbidity and color, pathogenic organisms, toxic chemicals, organic matter, and carcinogenic pollutants. This study aimed at investigating the Heterotrophic plate count (HPC) in drinking water and possibility of biofilm formation in household...

متن کامل

An evaluation of sampling and laboratory procedures for determination of heterotrophic plate counts in dental unit waterlines.

BACKGROUND The high numbers of heterotrophic microorganisms that have been cultured from dental unit waterlines (DUWs) have raised concern that this water may exceed suggested limits for heterotrophic plate counts (HPCs). The main purpose of this investigation was to examine HPC variability in DUWs and to examine in detail the effect of laboratory processing of water samples on HPC values. ME...

متن کامل

Quantidisc and Yea Method Comparison: Recovery and Enumeration of Mesophilic Heterotrophic Bacteria in Potable Water

Historically, heterotrophic plate counts are enumerated using a pour plate method, whereby plates are read manually. This analysis is important to evaluate the maintenance of water disinfection in a reticulation system. Advances in media and technology have made it possible to do this evaluation using defined substrate media and enumeration is performed by means of instrumentation using softwar...

متن کامل

Comparison of Conventional Plating and Simplate Methods for Enumeration of Aerobic Microorganisms, Coliform and Escherichia Coli in Farm Environmental Samples

The objective of this study was to compare conventional plating to SimPlate methods for enumeration of aerobic microorganisms, coliforms and Escherichia coli in samples from farm animals and their environment. Samples evaluated included rectal swabs, fecal material, bedding, litter and feed and soil from beef and dairy cattle, swine and poultry farms. Standard method agar pour plating and conve...

متن کامل

A faster and more economical alternative to the standard plate count (SPC) method for microbiological analyses of raw milks

According to the International Dairy Federation, the quality of raw milks shall be evaluated by the determination of "total" bacterial counts, or standard plate count (SPC); after serial dilution of the milk samples, the SPC is determined on Plate Count Agar (PCA) by the "pour plate" method; the viable bacteria are enumerated after aerobic incubation for 2 or 3 days, at 32 °C or 30°C respectively.

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Applied and environmental microbiology

دوره 66 1  شماره 

صفحات  -

تاریخ انتشار 2000